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Dodecamethylcyclohexasiloxane (D6) is widely used daily in the chemical
industry and exists in the environment; however, its eco-toxicity is not
well documented. A hydroponic experiment was performed to investigate
the effects of D6 exposure (10–1000 mg L−1) on oxidative stress induction and gene expression changes in crayfish (Procambarus clarkii). The results showed that superoxide dismutase (SOD) activity was enhanced by 20–32% at low D6 exposure (10 mg L−1) in muscle but reduced in gill tissue at high D6 exposure (1000 mg L−1). High D6 (1000 mg L−1)
also increased catalase (CAT) and peroxidase (POD) activities in muscle
tissue by 14–37% and 14–45%, respectively, and the same trend was
observed in the carapace and gill tissue of crayfish. The
Malondialdehyde (MDA), ascorbate (AsA), and glutathione (GSH) contents
were increased by 16–31%, 19–31%, and 21–28% in the muscle of crayfish
under D6 exposure. Additionally, silicon (Si) content increased in three
organs (gill, carapace, and muscle) of crayfish. Related genes involved
in enzyme and nonenzyme activities were detected, and when crayfish was
exposed to D6, genes such as Sod3, Cat3, Pod3, and Gsh3 were up-regulated, while Asa3 and Oxido3
were significantly down-regulated in the muscle. The research results
help us to understand the toxicity of D6 in crayfish and provide a basis
for further research on the mechanism of D6-induced stress in crayfish
and other aquatic organisms.
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Keywords:
silicone; D6; antioxidation; transcriptome; crayfish
Sources: Sustainability 2021, 13(6), 3495; https://doi.org/10.3390/su13063495
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