"Happy World Tuna Day 2026

Let's celebrate this incredible marine creature and raise awareness about the importance of sustainable fishing. May we continue to protect our oceans and ensure a healthy future for tuna and all marine life. Enjoy a day full of appreciation and responsibility!"

Director, Dr. Md Muzammel Hossain, BCFRC

Dr. Md. Muzammel Hossain is a member of the American Chemical Society

Joining the American Chemical Society is more than just a membership—it's a commitment to lifelong learning, innovation, and ethical scientific practice. As members, we contribute to a community dedicated to advancing chemistry for the benefit of society. Embrace this opportunity to collaborate, inspire, and lead with integrity, knowing that your dedication can spark positive change and foster breakthroughs that shape the future." His member id number is 34597674. https://credentials.acs.org/b71f627a-3398-4f48-82b5-fcf42a68b8da#acc.C6bad4JG

Team for Jagannath University Research and Higher Study Society (JnURHSS)

 

Comparative Analysis: CRISPR-Based Diagnostics vs. Polymerase Chain Reaction (PCR)

Molecular diagnostics is undergoing a strategic transition from the established PCR "gold standard" toward CRISPR-based diagnostics (CRISPR-Dx). This shift toward precision pathology addresses critical barriers in clinical accessibility, moving high-fidelity testing to the front lines. While both modalities identify nucleic acids, their divergent operational requirements create a fundamental divide in deployment feasibility.

The following technical factors differentiate these two diagnostic modalities:

* Thermal Requirements: PCR necessitates complex thermal cyclers to manage rapid fluctuations (45°C–98°C). Conversely, CRISPR is isothermal—often integrating with amplification methods like RPA or LAMP to function at room temperature—which significantly reduces equipment costs and enhances portability.

* Specificity and SNP Resolution: CRISPR leverages the trans-cleavage (collateral) activity of Cas12 and Cas13 proteins for superior molecular precision. By utilizing crRNA programming and synthetic mismatches, it differentiates single-nucleotide polymorphisms (SNPs) with higher fidelity than traditional PCR.

* Sensitivity Dynamics: PCR maintains a consistent detection limit of 1–10 copies. Although CRISPR targets femtomolar (fM) to attomolar (aM) sensitivity, current field iterations like TMC-CRISPR face trade-offs, showing approximately 44% sensitivity compared to RT-PCR in low viral load (high Ct) specimens.

* Workflow Complexity: PCR demands a controlled, unidirectional lab setup, highly skilled technicians, and multi-hour protocols. CRISPR platforms, such as SHERLOCK and DETECTR, enable simplified "one-pot" or pre-amplification-free protocols, delivering results in under one hour.

These differentiators dictate the global health landscape. CRISPR-Dx is the optimal solution for resource-limited, rural clinics where PCR infrastructure is logistically impossible. However, clinical consultants must weigh CRISPR's decentralized speed against the superior analytical sensitivity of laboratory-based PCR.

#CRISPR #crisprcas9 #PCR #biotechnology #ecogenezap 

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